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However, when the bacteria were treated with TA (5 mg/mL), PTA (10 mg/mL), and PTK (10 mg/mL) hydrogels, obvious red fluorescence (dead bacteria) was observed 12 h after incubation, which was attributed to the presence of TA in the hydrogel matrix 24,45

de Ruijter TC, Veeck J, de Hoon JP, van Engeland M, Tjan-Heijnen VC

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GHK-Cu response assessment should occur after minimum 12-16 weeks, accounting for hairs natural growth cycle

The cumulative nature of the treatment means consistency over months matters more than any single application