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Metabolite set enrichment analyses were performed with the platform MetaboAnalyst (33) ( Gene expression analysis Cell lines (5 to 10 10 6 cells) in the exponential growth phase were maintained overnight in fresh culture medium and then had their RNA extracted using the Illustra RNAspin Mini Kit (GE Healthcare Life Sciences, Pittsburgh, PA, USA)

PAT formation occurs through transdifferentiation, where white adipocytes undergo significant phenotypic and functional changes, including the development of milk-producing capabilities and alterations in lipid storage and secretion (21)

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Collagen gel contraction assays Fibroblast cultures at passage 3 were cultured in six-well tissue culture plates (Gibco, Canada) in growth medium (DMEM, 10% FBS, penicillin, and streptavidin from Invitrogen, Canada)

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[2] The lower headline number in REDEFINE 2 than in REDEFINE 1 is the expected pattern weight loss is typically smaller in populations with type 2 diabetes and is a good reminder that a trials population matters as much as its drug