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After centrifugation to pellet non-dissolved tissues, the supernatant was filter sterilized (0.2 m) and used for total protein quantification using the BCA method (Thermo Scientific)

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Higher triglycerides were associated with lower glutathione concentration in our data and this may be consistent with an accelerated lipolysis theory

OH may damage biological components including as lipids, proteins, DNA, and membranes

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2i and 2j, the HF diet-feeding resulted in an increase in serum insulin and leptin levels in the mice, which were markedly reduced by PPT treatment