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Thermostability ( T 50 ) The kinetic thermostability of purified LacAnc100 and LaccID was estimated by assessing their T 50 values using 96-well gradient thermocyclers (T100 thermal cycler, Bio-Rad)

Based on the distribution, a two-tailed Students t-test, paired Students t-test, Mann-Whitney test, or Wilcoxon test were employed to compare the groups

For decades, research has centered on glucose and lipid metabolism disorders (e.g., insulin resistance and hyperlipidemia) as central drivers of metabolic diseases ( As an essential AAA, phenylalanine (Phe) metabolism is predominantly governed by hepatic phenylalanine hydroxylase (PAH) ( Interestingly, gut microbiota-derived Phe metabolites, such as phenylacetylglutamine (PAGln) and exercise-induced N-lactoyl-phenylalanine (Lac-Phe), demonstrate diverse and beneficial bioactivities in cardiovascular risk modulation and appetite regulation, respectively ( Therefore, understanding the mechanisms by which dysregulated Phe triggers metabolic dysfunction is crucial

Check the osmotic pressure with an osmometer if possible

10.1016/j.jplph.2007.01.017 30 EltelibH

This research was financially supported in part by grants from the National Natural Science Foundation of China (31370332), Plan for Scientific Innovation Talent of Henan Province (144200510017), National Key Research and Development Program (2016YFD0101006) and the Ministry of Agriculture of China (2016ZX08009-003)