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Immunofluorescent staining Cells were washed with PBS at 1000 rpm for 5 min twice, fixed with 4% paraformaldehyde for 2 h, then permeabilized with 0.1% Triton X-100 for 10 min at room temperature

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(C) Typical examples of resting, ramified microglial cell (top) and reactive, amoeboid cell (bottom)

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