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Description

Amino acids V12 (putative oxyanion hole), F112 (active site architecture), N108 (flavonoid binding) and C13 (GSH stabilization) were fully randomized by site saturation mutagenesis (NNK codons), and the created Pt GSTF8 variants were tested for conversion of flavan-3,3,4-triol ( 4 ) to cyanidin ( 8 ), and for their GSH-transferase activity towards CDNB ( 5 ) as a control of catalytic competence

PerutFRoncuzziLAvnetSMassaAZiniNSabbadiniSet al

A.ChelmowD.DaveyD

10.3389/fphar.2014.00151 Front

These results confirmed that ROS and the excitatory potency of acidic amino acids such as glutamate are involved in methylmercury-induced neurotoxicity in cerebral neuron cultures