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2.5 RNA isolation and quantitative real-time PCR Total RNA was extracted from HCECs using the TRIzol reagent (Vazyme Biotech Co., Ltd., Nanjing, China), according to the manufacturers instructions

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[DOI] [PMC free article] [PubMed] [Google Scholar] 62.Ramirez-Exposito M.J., Carrera-Gonzalez M.P., Mayas M.D., Martinez-Martos J.M

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