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10.1038/nature02866 14 WadaJ.MakinoH

For immunohistochemistry (IHC), antigen retrieval was performed on rehydrated sections and sodium citrate buffer (10 mM, pH 6.0) in a pressure cooker at high pressure for 5 minutes was used for F4/80, while Tris-EDTA buffer (10 mM, pH 9.0) at high pressure for 10 minutes was used for PCNA and Ly6g

For the initiation phase, where you need 1-2mg, reconstituting at 5mg/mL or 10mg/mL is far more accurate

Practical Considerations Favoring Oral Delivery: Beyond bioavailability questions, oral administration offers significant lifestyle advantages that make peptide therapy accessible to a broader population: No Injection Requirement: Many individuals interested in tissue support supplementation are deterred by injection protocols

Consent was obtained from all participants

mild soreness for a day or two is normal