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The streptavidin sensor chip was preconditioned with 1 M NaCl and 40 mM NaOH (60 s 3 cycles, 100 L/min), running buffer (60 s, 100 L/min), and functionalized to capture His-tagged proteins by 350 mM EDTA (60 s), 1 M TrisNTA biotin trifluoroacetate salt solution (SigmaAldrich 75543) (100 s), 500 M NiCl 2 (100 s), 3 mM EDTA (60 s) (for all functionalizing steps: flow rate 100 L/min, reagents in running buffer)

1):S14246

Okamoto, Y