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10.1016/j.kint.2016.01.014 53 KinugasaY.NakamuraK.KamitaniH.HiraiM.YanagiharaK.KatoM.et al (2021)

Also known as cobalamin, is a water-soluble vitamin that has many important functions throughout the body, including keeping nerves healthy, supporting the production of DNA and red blood cells, and maintaining normal brain function

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To further assess target engagement in vitro, we used a competitive fluorescence polarization (FP) assay that reports on the displacement of pomalidomideBODIPY from purified preparations of recombinant CRBNDDB1 (ref

2.9 Seahorse XF Mito Stress Test assay MEC-1 and HG-3 cells were seeded at a density of 1 10 6 cells/mL and incubated for 24 hours with DMSO and CB-839 at equitoxic concentrations based on the IC 50 values established during the MTS assay

Stock solutions were prepared and dissolved following manufacturers instructions