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Nile Red staining of fixed worms is a better indicator of fat stores than Nile Red staining of live worms Because we found that wild-type worms grown on various bacterial strains do not show a difference in staining pattern visualized with Nile Red staining of live worms, but do have significant differences in fat stores as measured by TLC/GC lipid quantification or visualized with Nile Red in fixed worms, we examined a number of mutants that had previously reported fat storage differences measured by the levels of Nile Red brightness in live mutants

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These fasted mutants also exhibited physiological changes similar to those observed in wild-type fasted animals, including reduced body size and enhanced motility (Supplementary Fig

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Profiles Dr Tim Jackson Consultant in Palliative Medicine Northern Care Alliance NHS Trust Dr Tim Jackson has been a consultant at Salford Royal Hospital and St