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The analysis for the PAD-exposed tau (red) channel was run with the following settings: pixel intensity threshold of 15,000, background set to dark, split touching objects = yes, fill holes in mask = yes, no background flattening, image smoothing strength was 0, evaluate background on 5% of lowest pixels, minimum and maximum object size were set to 0.1 m and 100 m, respectively, and primary edge objects were included

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2I), despite the alteration in both the number and morphogenesis of DCX-positive immature neurons observed under diabetic conditions

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