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Institutional Review Board Statement The study was conducted in accordance with the Declaration of Helsinki, and approved by the Research Ethics Committee of Ain Shams University (protocol code ACUC-FP-ASU-RHDIRB20202110301 REC NO

3H) which overlaps with a previously established USP11 UBL binding site for a AEGE F YK L KIRTPQ peptide (PDB: 5OK6

B.1994Glycine and taurine conjugation of bile acids by a single enzyme

The membrane was washed with TBS three times at room temperature, shaking for 10 min

The chemical reactions which occur in the bisulfite sequencing protocol result in altering and degrading the DNA, providing only short strands for analysis, and therefore not being used for long-read sequencing [71]

Inactivation of the IGF-I gene in mice results in perinatal lethality