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The cofactor (PLP) content was measured by treating TgCBS with 0.1 M NaOH and monitoring absorbance at 388 nm ( 388 = 6,600 M 1 cm 1 ) 45,46

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Thyroid hormones as modulators of immune activities at the cellular level

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In the yeast enzyme, an active site Mg 2+ , orients the glutamate -carboxylate for attack on the -phosphate of ATP, which is positioned by a second Mg 2+ and an active site lysine