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Metagenomic chromosome conformation capture (Meta3C) libraries generation and sequencing Frozen fecal samples were thawed, and 500 mg of each sample was resuspended in 200 mL of crosslinking solution of 1 PBS with 5% formaldehyde (Sigma AldrichFormaldehyde 36.5% in Methanol 15%) and incubated for 1 h at RT under gentle agitation

doi: 10.1590/S0365-05962013000100009 36 WalmsleyR.BarrettM