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Grant JE, Kim SW, Odlaug BL

To tease out the interplay between mTORC1 and autophagy in responding to these inputs, we used cultured rat (L6) myotubes, exposed to supplemental growth factors, leucine, or electrical pulse stimulation, to investigate the effects of each of these anabolic stimulators in the presence or absence of the ATG4B inhibitor NSC185058

These tissues respond dynamically to nutrient levels, hormonal cues, especially insulin, and the cellular energy status, converging on mTOR complexes to determine whether cells undergo anabolic growth or catabolic breakdown (Fig

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In these derivatives, the iron-binding activity has been significantly reduced