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Plasma glutathione is typically lower (15 mol/L) and reflects acute oxidative demand

The graphical analyzis of Figure 2F also suggests a trend towards a reduction in GSSG in the symptomatic group, but without statistical significance (29.79 15.40 pmol mg protein -1 and 72.66 66.84 pmol mg protein -1

MS Grade Trypsin Protease (Thermo Fisher Scientific) was added at a 1:20 enzyme-to-protein ratio (w/w), and digestion was carried out at 37 C for 16 h