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Quantitative real-time PCR (qRT-PCR) was conducted using the ABI Viia 7 Real-Time PCR System (Applied Biosystems, USA) with ChamQ SYBR qPCR Master Mix (Vazyme, China)

Interestingly, they also compared the abovementioned DNA-TG cutoff level with a 6-TGN cutoff level ( 355 pmol/8 10 8 RBC) from literature based on patients from Asian descent [45]

Phosphomannomutase 2 (PMM2)-CDG, Glucosyltransferase 1 (ALG6)-CDG and Mannosyltransferase 6 (ALG3)-CDG are complex disorders with multiorgan involvement and can present with HI

& Frolov, M