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After washing for 5 min with 0.1% formic acid (v/v) and 3% acetonitrile (v/v) at a flow rate of 10 l/min, the integrated switching valve was activated and peptides were eluted in the back-flush mode from the extraction column onto a 150 0.075 mm C18, 2 m resin column (GlycoproSIL C18-80, Glycopromass)

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