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It was also found that ME/CFS patients are unable to dilate their vessels adequately by endothelium-independent vasodilation when given sublingual nitroglycerin to promote relaxation of the vessels

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Furthermore, insulin resistance can lead to abnormal lipid profiles, like low-HDL and high triglyceride (TG) levels

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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig