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Evaluation of the neuroprotective activity of PEDF in Y79 cells Zinc stress of Y79 cells was induced by adding 100 M ZnSO 4 to FBS-free medium and culturing cells for 2, 4, 6, or 8 h as described above, except that 15 min before zinc treatment, purified recombinant PEDF in 20 mM Tris-HCl, pH 8.0, was added to the medium to a final concentration of 1 M

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The convergence of phenological advancement and altered beach thermal regimes thus creates compounding physiological risks that cannot be fully mitigated through behavioral adjustment alone, underscoring that reptile phenological responses involve complex demographic trade-offs that deserve substantially greater research attention

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