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vitamin E When it comes to antioxidative effects, vitamin C primarily neutralizes water-soluble oxygen free radicals, like hydroxyl radicals and singlet oxygen, which protect the cellular environment ( Vitamin E, on the other hand, acts mainly on lipid layers, stabilizing cell membrane structures and preventing lipid peroxidation damage

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After putting the finished reaction mixture in a thermal cycler, the following procedure was run: reverse transcription for 30 min at 50 C, primary denaturation for 10 min at 94 C, 40 cycles of 94 C for 15 s, annealing temperatures for 1 min as indicated in Table 5, and 72 C for 30 s