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Description

We mixed 5 L of the 1st supernatant obtained with 45 L of isotopically labeled internal standards (d 3 -carnitine, d 9 -TMAO, and 13 C 3 -TMAO) in 0.1% formic acid acetonitrile solution

Metabolite-metabolite and metabolite-gene interaction analysis To further explore the functional relationships among metabolites and their potential links to gene regulation, metabolitemetabolite and metabolitegene interaction networks were constructed using plasma metabolomic data from ASD and healthy control participants (Fig