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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

Skin Regeneration GHK-Cu enhances skin renewal by promoting cell turnover and supporting tissue repair

Advances in dietary, pharmacological, and genetic approaches targeting lipid metabolism and iron homeostasis further underscore its therapeutic potential

10.1039/d0lc00981d 74 ZhangL.XueY.MuD.ChenS.WuG.YangZ.et al (2025)

HIF-1 levels surged significantly (519%) after IR damage

The absorbance was measured at 340 nm for 5 min, with readings every minute