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Description

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Mice were intraperitoneally injected with pregnant horse serum gonadotropin (10 IU, Ningbo Sanseng Pharmaceutical Co., Ltd, Zhejiang, China)

All statistical analyses were performed using the SPSS 17.0 software (Chicago, IL, USA)

Int J Oncol 44:830837

Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)