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The 8-OHdG levels in the H 2 group were significantly suppressed immediately, 3 h and 24 h after the race compared to in the placebo group

Co-Immunoprecipitation for immunoblot analysis A total of 1 10 7 HaCaT cells per condition were lysed in 2 ml of 1% Igepal buffer (1% Igepal CA-630, 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 10% glycerol, 10 g/ml leupeptin, 10 g/ml aprotinin and 0.5 mM AEBSF)

doi:10.1016/S0021-9258(19)75968-7

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Uridine alleviating the need to increase mitochondrial biogenesis led us to conclude that increased pyrimidine salvage (probably via UCK2) is driving this mitochondrial adaptation

Figure 4 illustrates the key mitochondrial antioxidant defense systems against ROS