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DNA fiber assay Cells were pulse-labeled with 30 M thymidine analog 5-chloro-2-deoxyuridine (CldU) (Sigma-Aldrich, C6891) for 30 min, then washed three times with PBS (pre-warmed to 37 C), followed by pulse-labeling with 250 M 5-iodo-2-deoxyuridine (IdU) (Sigma-Aldrich, I7125) for 30 min

Am J Cancer Res 10(12):4464-4475, 2020

Gosk S, Vermehren C, Storm G, Moos T (2004) Targeting anti-transferrin receptor antibody (OX26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion

Figure 2 3.1 Activation of inflammatory signaling pathways Research indicates that increased intestinal permeability allows endotoxins such as LPS to enter the systemic circulation, where they trigger systemic inflammatory responses by activating the Toll-like receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells (TLR4/NF-B) inflammatory pathway

Pfeiffer, R

It prevents metabolic impairment reducing hepatic inflammation