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2.5 RNA isolation and quantitative real-time PCR Total RNA was extracted from HCECs using the TRIzol reagent (Vazyme Biotech Co., Ltd., Nanjing, China), according to the manufacturers instructions

Consuming pure cysteine is ineffective because it is easily oxidized and poorly absorbed, but NAC effectively bypasses liver metabolism, crossing the blood-brain barrier to deliver L-cysteine directly to the cells