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The blots were washed and then incubated with a secondary antibody conjugated to horseradish peroxidase (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and the blots were visualized using a western blotting luminol reagent kit (Santa Cruz Biotechnology, Santa Cruz, CA.)

Therefore, suppressing NCOA4 can inhibit the degradation of ferritin and the occurrence of ferroptosis ( Ferroptosis and Cancer Stem Cells Hallmarks of CSCs CSCs are a small section of tumor cells that possesses the ability to self-renew, initiate tumors, and cause resistance to conventional anticancer agents

A liver-derived secretory protein, selenoprotein P, causes insulin resistance

Amino acids are linked to each other by peptide bonds, in which the carboxyl group of one amino acid is joined to the amino group of the next, with the loss of a molecule of water

Kobayashi et al., 2019