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Standard protocols suggest 4 weeks of active use followed by 2-4 weeks of rest

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The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord

For replating, HBs were dissociated using Accutase and reseeded onto Matrigel-coated plates