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Our approach enabled us to test four different hypotheses (Table 1) regarding the impact of preparation conditions on plasma GSH measures

Despite an increasing number of therapies available in the IBD clinic, optimisation of each therapy before switching within or out of class remains good medicine

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The enzyme that recycles BH2 is DHFR.29 However, DHFR has another important job

An oxidation pathway, in which jasmonoyl-isoleucine is oxidized by cytochrome P450 enzymes, mainly CYP94 isoforms, to a 12-hydroxy metabolite and then further via a 12aldehydo intermediate to dicarboxy-jasmonoyl-isoleucine, together with deconjugation by amidohydrolases, is now recognized as a major route for catabolism and deactivation of the hormone, although the hydroxy intermediate can itself cause weak and sustainable expression of certain JA-responsive genes until further oxidation occurs