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A PCR master mix was employed with final concentrations of Standard ThermoPol buffer Mg-free (1x) (New England Biolabs, Ipswich MA, USA), dNTPs (0.2 mM each) (Bioline, London UK), MgCl2 (1.5 mM) (Bioline, London UK), 0.5 M each of forward and reverse primers, 0.625 units of Immolase Taq polymerase (Bioline, London, UK), 2 L genomic DNA and PCR-grade H2O, to a final volume of 25 L

The Wnt Signaling Pathway Effector TCF7L2 Controls Gut and Brain Proglucagon Gene Expression and Glucose Homeostasis

It also binds hormone receptors, including ER, to inhibit hormone-induced apoptosis

J., Mastick, C

CYY assisted in conducting in vivo experiments and acquiring data

Tekautz et al