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10.1111/j.1744-7909.2007.00583.x 82 LundeC.BaumannU.ShirleyN

IC50, a concentration that causes 50% inhibition

It was recently revealed that the ferroptosis inducers sorafenib and erastin promote cuproptosis by enhancing copper-dependent lipoylated protein aggregation in primary liver cancer cells [129]

Then, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.02% Triton-X-100/1 phosphate-buffered saline (PBS) and blocked in a blocking fluid (1 PBS + 10% fetal bovine serum (FBS) and 1% bovine serum albumin (BSA)) (Thermo Fisher Scientific, Waltham, USA)