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What If Water Retention Becomes Severe Enough to Interfere with Data Collection

4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk, and ECL (GE Healthcare)

Since GSH binding has been shown to stabilise PV 17,23 , we next used the PaSTRy assay 24 to demonstrate that GSH also shows a modest dose-dependent stabilisation of EV-F3 (Methods, Supplementary Fig

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