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Differential effects of E-cigarette on microvascular endothelial function, arterial stiffness and oxidative stress: a randomized crossover trial

qRT-PCR Total RNA was isolated separately from the apical and basal chamber and subsequently purified using the Direct-zol RNA Microprep kit (Zymo Research, Switzerland) using the manufacturer protocol

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Treatment optimization : this two-pronged approach can sometimes restore efficacy comparable to that observed in early trials with tadalafil alone

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