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Briefly, we allowed 3 mismatches in the 18 bp invariant sequence upstream, and 4 mismatches in the 18 bp downstream of the transposon barcodes using a Hamming distance function to compare the expected and actual sequences

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5 L of each sample was analysed on a Acquity UPLC HSS-T3 column (50 2.1 mm, 1.8 m, Waters, MA, USA) by using gradient elution of 0.1% formic acid ( v / v ) in water as mobile phase A and 0.1% formic acid ( v / v ) in acetonitrile as mobile phase B, with a flow rate of 0.4 mL/min with the following gradient: 00.5 min (0% B), 0.8 min (15% B), 1.2 min (80% B), 1.4 min (80% B), 1.5 min (0% B), 2 min (0% B)

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PCs modulate glucose metabolism by regulating microRNA expression in the pancreas (Liu et al