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n = 5 mice per group

Force curves were collected by approaching the functionalized tip on different points of the substrates surfaces at the constant velocity of 50 nm/s, while the retraction velocity was varied from 50 to 4,200 nm/s, according to the selected nominal loading rates, defined as the product of the nominal cantilever spring constant ( k nom ) by the tip pulling velocity ( v ), and set in the nominal range of 184 nN/s

PubMed, Santos, Beatriz Werneck Lopes, et al

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Therefore, by transfecting M cell lines with targeted Apollo-NADP + sensors and treating cells with 2-DG and diamide, we can measure NADPH consumption by GSR in the cytosol or mitochondria in a kinetic assay