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The cells were evaluated using a full-wavelength multifunctional microplate reader (Tecan, Mnnedorf, Switzerland)

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GSTP1 was subjected to a series of chemical treatments pre- or post-palmitoylation in vitro and resolved on an 11% SDS-PAGE gel and transferred to a PVDF membrane

Notably, inhibition of MAPK/ERK signalling exacerbates 3-HK-induced mitochondrial dysfunction and cell death, implicating impaired cellular resilience pathways in amplifying KYN-mediated toxicity

Zhiyuan Zhang, Jiaru Gao and Linjiao Jia contributed equally to this work