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Unable to obtain or design primers specifically targeting esr2b (ER1), we performed Western blots with retinal homogenates using a general esr2 (ER) antibody to potentially reveal ER isoform-specific differences in expression when compared with qPCR data

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Neuroscience Research, 31(4), 289-305

The tissue sections were then processed by blocking the endogenous peroxidase activity for 10 min, followed by incubation with primary antibodies