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In-Vitro Research Considerations For in-vitro work, key variables include cell line selection, serum conditions, vehicle controls, and peptide concentration ranges

Filtered through a 100 m nylon mesh and centrifuged at 50 g for 5 min, resuspended cells with 5 mL medium and then added 5 mL gradient solution (10PBS: Percoll=1:9, v/v), gently pipetted to mix, centrifuged at 120 g for 5 min, removed the floating particles and supernatant

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Left hippocampus in patients were smaller than that in controls ( P= 0.002), whereas right hippocampus difference did not reach statistical significance

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Expression of GSTP1 prevented QI-induced activation of caspase 3 almost completely