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Following treatment, media was replaced with 80% (v/v) methanol (cooled to 80C) containing 500 nmol 6-chloronicotinamide (6-CN) as an internal standard (IS) to extract cellular metabolites

Another intriguing MGBA aspect in AD is the direct effect of gut microbes on amyloid and tau pathology

The metabolite -KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8