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Gastroprotection Gastric mucosa Protection and healing of gastric ulcers, colitis, and intestinal lesions in animal models

Cell culture and stimulation Normal human epidermal keratinocytes (NHEKs) were purchased from Takara Bio (Shiga, Japan) and cultured in Keratinocyte Basal Medium (KBM) overnight prior to human recombinant rIL-22 stimulation (200 ng ml 1 , R&D Systems, Minneapolis, MN)

It dissolves well in DMSO at 1mg/ml However, adding water may cause cloudiness In that case, applying ultrasonic treatment for 2 seconds usually clears the solution Consequently , proper handling ensures accurate experimental results and consistent performance