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Metabolism of non-transferrin bound iron produces reactive oxygen species that contributes to the cellular dysfunction and apoptosis of enterocytes [53] and results in damage to the TJ complexes and leakage of LPS and (1 3)--d-glucan (BG), a major component of the fungal cell wall into the circulation [54]

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Mice euthanized with 300 mg/kg sodium pentobarbital were perfused with a 0.9% saline solution, the eyes were removed, and the retinas rinsed in ice-cold saline, weighed, and then homogenized in Tris buffer (pH 7.4) containing BHT to prevent sample oxidation