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The lipid residue in the LC-MS injection vial was redissolved with 300 L of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20C for 2 h

The rapidity with which an addicted smoker can achieve sufficient central nervous system (CNS) levels of nicotine from inhaled tobacco smoke to remove any withdrawal effects is another example

mBio (2019) 10(2)

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Patent No 05485371

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