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Deep penetration of FeS@SRB in 3D tumor spheroids FITC-labeled FeS@SRB were obtained by co-incubation of FTIC and FeS@SRB at 37 C for 15 min

The expression of SR-BI on macrophages post different treatments was evaluated by immunofluorescence staining using anti-SR-BI antibody (incubated overnight at 4 C) and Alexa Fluor 488-conjugated secondary antibody (incubated for 1 h at room temperature)

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It has been reported that trastuzumab resistant cells exhibit enhanced glycolysis phenotype, and glycolytic restraint is able to sensitize trastuzumab resistant HER2+ breast cancers to trastuzumab treatment (303)

The membrane was blocked in blocking buffer (5% BSA in 1TBST, Tris-buffered saline and Tween-20) and then incubated with primary antibodies overnight at 4 C

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