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A real time-PCR analysis was conducted using SYBR Premix-Ex TagTM (Takara, Dalian, China) on a Roche LightCycler 480 System (Roche Applied Science, Penzberg, Germany)

The upper phase was evaporated under nitrogen and re-dissolved in toluene and vortexed thoroughly

Insulin-like growth factor-1 supplementation promotes brain maturation in preterm pigs

Sample collection After eight weeks, the rats in the control and experimental groups were fasted overnight for 1618 h with free access to water to reduce the post-prandial variability of liver enzyme activities

This protective effect may be mediated through the STAT3 signaling pathway (Figure 5)

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