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Immunological studies typically use membrane-incorporated dyes, or genetic switches that result in constitutive fluorochrome expression if a cell marker is expressed

In addition, a study conducted in BV2 murine microglia cells, which served as a representative cell specimen for brain microglia, showed that the upregulation of antioxidant/detoxification proteins by SFN was associated with enhanced resistance of microglial cells to heat-induced toxicity and removal of ROS during microglial cell activation (90)

After washing with TBST, the membranes were treated with secondary antibody (Proteintech) for 2 h at room temperature

Moreover, T cells, DCs, and Tregs secrete another type of antioxidant, TRX, which preserves the reduced form of thiols on the cell surface (84)

The mitochondrial membrane transport reactions included were pyruvate transport (that represents the pyruvate-proton (Pyr - /H + ) cotransporter) as well as Mal/Suc, Mal/2-oxo and Mal/Iso exchangers ( Supplementary Table S1 )