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Vascular wall cells contribute to tumourigenesis in cutaneous neurofibromas of patients with neurofibromatosis type 1

Transcriptional profiling by RNA sequencing (RNA-seq) of HSCs isolated from 2-year-old Mx1-T3 and Mx1-Cre control mice showed that SIRT3 overexpression increased expression of mitochondrial genes, including Nd4 , Cytb , Nd1 , Cox7a2 , Ndufa6 , Tomm7 , Nd2 and Timm8b (Extended Data Fig

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Mice infection C57BL/6 female mice, CD4 Cre Ifng fl/fl female mice, CD4 Cre WDR24 fl/fl and Rag1 / female mice (68 weeks old) were divided randomly into cages and infected using an aerosol method with ~200 c.f.u.s of different H37Rv strains for 1 day or 4 weeks (using an inhalation exposure system from Glas-col) at the Biosafety Level-3 (BSL-3) Laboratory

Its action is specific to adipocytes (fat cells) via beta-3 adrenergic receptors and does not affect anabolic pathways, protein synthesis signaling, or IGF-1 levels

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