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Association of TNF- promoter polymorphism and Graves' disease: an updated systematic review and meta-analysis

All the values were normalized against the housekeeping gene (-actin) with the delta-delta Cycle Threshold (DDCT) method

Figure 5: The technological process of extraction of glutathione from yeast cell 1.Reduced glutathione is a kind of small molecular peptide, a large number of peptides in living organisms, especially in liver cells, protect the liver cell membrane, promote the role of liver enzyme activity, and with a number of toxic chemicals in combination with play the role of detoxification.To the drug poisoning, alcoholism and other causes of liver injury, disease such as cirrhosis of the liver have good curative effect

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Cells were washed with washing buffer [BSA (0.3%) + Triton (0.1%) in 1X PBS] and from this step on, reagents from the DUOLINK PLA kit were used according to the manufacturers instructions (OLINK Bioscience, Uppsala, Sweden)