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The supernatants were collected and the amount of TNF-, IL-6, and VEGF secreted were measured by ELISA kits (KeyGEN, China)

After adding 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) to a final concentration of 10 mM and setting the pH to 6.2 using 5 N NaOH, the product was extracted four times with 1 vol of ethyl acetate

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The patient usually relaxes and listens to music or watches videos as the treatment is being performed and experiences little to no discomfort

First, air bubbles in the syringe barrel displace liquid volume